LSM 780 NLO (C3.211)

Microscope

Inverted Zeiss LSM 780

2016-01-18 11.56.26

Lasers

  • 1-photon
    • diode 405                                                            (DAPI)
    • Argon 458, 488, 514                                         (CFP, GFP, YFP)
    • HeNe 543                                                            (RFP)
    • HeNe 633                                                            (Far red)
  • 2-photon
    • Chameleon Vision II (690-1064 nm)            (Dapi, CFP, GFP, YFP, RFP)

Other light sources
(only via oculars, no recording)

  • HALOGEN for brightfield transmitted light
  • LED illumination (CoolLED) for epifluorescence (blue, green, red emission)

Objectives (See Objective Guide)

  • Plan Apochromat 10x/0.45 M27 (WD 2000 um)
  • Plan Apochromat 20x/0.8 M27 (WD 550 um)
  • C Achroplan 32x/0.85 W Korr M27 (WD 1100 um)
  • LD C Apochromat 40x/1.1 W Korr M27 (WD 620 um)
  • alpha Plan Apochromat 63x/1.46 Oil Korr M27 (WD 190 um)

Software

ZEN 2012 SP1 (64-bit) release 8.1
Extra modules: TILES, Multiple time series, MACRO

Filters


Detectors

  • internal PMT 1
  • internal GaAsP detector array (32x)(spectral imaging possible)
  • internal PMT 2
  • external BiG GaAsP detector (2 channels)
  • T-PMT (transmitted light)

Also available

  • Motorized stage
  • Incubator
  • CO2 control

Documents:

  • start up (Link) and shut down (Link) procedures